Anti-TREM1 / CD354 Reference Antibody (PY159)

Recombinant Antibody

Anti-TREM1 / CD354 Reference Antibody (PY159) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%
The purity of Anti-TREM1 / CD354 Reference Antibody (PY159)is more than 95% ,determined by SEC-HPLC.
Immobilized human TREM1 His at 2 µg/mL can bind Anti-TREM1 / CD354 Reference Antibody (PY159)，EC50=0.00645 µg/mL
Human TREM1 HEK293 cells were stained with Anti-TREM1 / CD354 Reference Antibody (PY159) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC275=0.7744nM
The endocytosis ratio PY159 by hu-TREM1-HEK293 increased with the increase of antibody concentration, and the Internalization Rate (%) reached 80% at antibody concentration of 0.3 nM.
Anti-TREM1 Reference Antibody (PY159) Activation was evaluated using PBMC. The max induction fold was approximately 3.16

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	FC, Kinetics, Animal Model
Primary Accession	Q9NP99
Reactivity	Human
Clonality	Monoclonal
Isotype	IgG1
Calculated MW	143 KDa

Additional Information
Target/Specificity	TREM1 / CD354
Endotoxin	< 0.001EU/ µg,determined by LAL method.
Conjugation	Unconjugated
Expression system	CHO Cell
Format	Purified monoclonal antibody supplied in PBS, pH6.0, without preservative.This antibody is purified through a protein A column.