Anti-MASP2 Reference Antibody (narsoplimab)

Recombinant Antibody

Anti-MASP2 Reference Antibody (narsoplimab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%
The purity of Anti-MASP2 Reference Antibody (narsoplimab)is more than 98.94% ,determined by SEC-HPLC.
Immobilized human MASP 2A His at 2 µg/mL can bind Anti-MASP2 Reference Antibody (narsoplimab)，EC50=0.006278 µg/mL
C5b-C9 of the MBL pathway can be completely inhibited by Anti-MASP2 Reference Antibody (narsoplimab) with an IC50 of 0.7337 µg/mL.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	FC, Kinetics, Animal Model
Primary Accession	O00187
Reactivity	Human, Mouse, Rat
Clonality	Monoclonal
Isotype	IgG4
Calculated MW	75702 Da

Additional Information
Target/Specificity	MASP2
Endotoxin	< 0.001EU/ µg,determined by LAL method.
Conjugation	Unconjugated
Expression system	CHO Cell
Format	Purified monoclonal antibody supplied in PBS, pH6.0, without preservative.This antibody is purified through a protein A column.

Protein Information
Name	MASP2 {ECO:0000303\|PubMed:9087411, ECO:0000312\|HGNC:HGNC:6902}
Function	Precursor of a serum protease that activates the lectin pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed:11527969, PubMed:22691502). The lectin complement system is activated following association of lectins, such as MBL2, FCN1, FCN2 or FCN3, to carbohydrates on the pathogen surface (PubMed:22691502, PubMed:22966085). MASP2 is cleaved and activated by MASP1 in response to lectin-binding to pathogen carbohydrates (PubMed:10946292, PubMed:22949645, PubMed:22966085, PubMed:9087411). Can activate prothrombin to thrombin (PubMed:39924859).
Cellular Location	Secreted.
Tissue Location	Plasma..