Anti-CDH17 / Cadherin-17 Reference Antibody (10C12)

Recombinant Antibody

Anti-CDH17 / Cadherin-17 Reference Antibody (10C12) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%
The purity of Anti-CDH17 / Cadherin-17 Reference Antibody (10C12)is more than 98.15% ,determined by SEC-HPLC.
Immobilized human CDH17, His Tag at 2 µg/mL can bind Anti-CDH17 / Cadherin-17 Reference Antibody (10C12)，EC50=0.00468 µg/mL
Human CDH17 HEK293 cells were stained with Anti-CDH17 / Cadherin-17 Reference Antibody (10C12) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC240=0.06889 µg/mL
The endocytosis ratio 10C12 by Human CDH17 HEK293 increased with the increase of antibody concentration, and the Internalization Rate (%) reached 80% at antibody concentration of 2 nM.
10C12 inhibited the tumor growth of COLO205 on nude mice. The result showed significant anti-tumor effects, with an tumor inhibition rate (TGI) of 70.0% at 5 mpk at D26.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	FC, Kinetics, Animal Model
Primary Accession	Q12864
Reactivity	Human
Clonality	Monoclonal
Isotype	IgG1
Calculated MW	143 KDa

Additional Information
Target/Specificity	CDH17 / Cadherin-17
Endotoxin	< 0.001EU/ µg,determined by LAL method.
Conjugation	Unconjugated
Expression system	CHO Cell
Format	Purified monoclonal antibody supplied in PBS, pH6.0, without preservative.This antibody is purified through a protein A column.