Anti-B7-H4 / VTCN1 Reference Antibody (Millennium patent anti-B7-H4)

Recombinant Antibody

Anti-B7-H4 / VTCN1 Reference Antibody (Millennium patent anti-B7-H4) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%
The purity of Anti-B7-H4 / VTCN1 Reference Antibody (Millennium patent anti-B7-H4)is more than 95% ,determined by SEC-HPLC.
Immobilized human B7 H4 at 2 µg/mL can bind Anti-B7-H4 / VTCN1 Reference Antibody (Millennium patent anti-B7-H4)，EC50=0.07145 µg/mL

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	FC, Kinetics, Animal Model
Primary Accession	Q7Z7D3
Reactivity	Human
Clonality	Monoclonal
Isotype	IgG1
Calculated MW	30878 Da

Additional Information
Target/Specificity	B7-H4 / VTCN1
Endotoxin	< 0.001EU/ µg,determined by LAL method.
Conjugation	Unconjugated
Expression system	CHO Cell
Format	Purified monoclonal antibody supplied in PBS, pH6.0, without preservative.This antibody is purified through a protein A column.

Protein Information
Name	VTCN1 {ECO:0000312\|EMBL:EAW56672.1}
Function	Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T- cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation.
Cellular Location	Cell membrane; Single-pass type I membrane protein. Note=Expressed at the cell surface. A soluble form has also been detected. {ECO:0000255, ECO:0000269\|PubMed:12818165, ECO:0000269\|PubMed:15878339, ECO:0000269\|PubMed:16782226}
Tissue Location	Overexpressed in breast, ovarian, endometrial, renal cell (RCC) and non-small-cell lung cancers (NSCLC). Expressed on activated T- and B-cells, monocytes and dendritic cells, but not expressed in most normal tissues (at protein level). Widely expressed, including in kidney, liver, lung, ovary, placenta, spleen and testis

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.