Anti-CXCR3 / GPR9 / CD183 Reference Antibody (Genzyme patent anti-CXCR3)

Recombinant Antibody

Anti-CXCR3 / GPR9 / CD183 Reference Antibody (Genzyme patent anti-CXCR3) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%
The purity of Anti-CXCR3 / GPR9 / CD183 Reference Antibody (Genzyme patent anti-CXCR3)is more than 100% ,determined by SEC-HPLC.
Immobilized human CXCR3 VLP Protein at 8 µg/mL can bind Anti-CXCR3 / GPR9 / CD183 Reference Antibody (Genzyme patent anti-CXCR3)，EC50=0.173 µg/mL

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	FC, Kinetics, Animal Model
Primary Accession	P49682
Reactivity	Human
Clonality	Monoclonal
Isotype	IgG1
Calculated MW	40660 Da

Additional Information
Target/Specificity	CXCR3 / GPR9 / CD183
Endotoxin	< 0.001EU/ µg,determined by LAL method.
Conjugation	Unconjugated
Expression system	CHO Cell
Format	Purified monoclonal antibody supplied in PBS, pH6.0, without preservative.This antibody is purified through a protein A column.

Protein Information
Name	CXCR3
Synonyms	GPR9
Function	[Isoform 1]: Receptor for the C-X-C chemokine CXCL9, CXCL10 and CXCL11 and mediates the proliferation, survival and angiogenic activity of human mesangial cells (HMC) through a heterotrimeric G- protein signaling pathway (PubMed:12782716). Binds to CCL21. Probably promotes cell chemotaxis response. Upon activation by PF4, induces activated T-lymphocytes migration mediated via downstream Ras/extracellular signal-regulated kinase (ERK) signaling. [Isoform 3]: Mediates the activity of CXCL11.
Cellular Location	[Isoform 1]: Cell membrane; Multi-pass membrane protein
Tissue Location	Isoform 1 and isoform 2 are mainly expressed in heart, kidney, liver and skeletal muscle. Isoform 1 is also expressed in placenta. Isoform 2 is expressed in endothelial cells. Expressed in T-cells (at protein level).

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.