Anti-CCN2 / CTGF Reference Antibody (pamrevlumab)

Recombinant Antibody

Anti-CCN2 / CTGF Reference Antibody (pamrevlumab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%
The purity of Anti-CCN2 / CTGF Reference Antibody (pamrevlumab)is more than 100% ,determined by SEC-HPLC.
Immobilized human CTGF His at 2 µg/mL can bind Anti-CCN2 / CTGF Reference Antibody (pamrevlumab)，EC50=0.006105 µg/mL.
Pamrevlumab inhibited the tumor growth of COLO205 on Balb/c nude mice. The result showed significant anti-tumor effects, with an tumor inhibition rate (TGI) of 39% at 40 mpk at D31.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	FC, Kinetics, Animal Model
Primary Accession	P29279
Reactivity	Human, Mouse, Rat, Rabbit
Clonality	Monoclonal
Isotype	IgG1
Calculated MW	38091 Da

Additional Information
Target/Specificity	CCN2 / CTGF
Endotoxin	< 0.001EU/ µg,determined by LAL method.
Conjugation	Unconjugated
Expression system	CHO Cell
Format	Purified monoclonal antibody supplied in PBS, pH6.0, without preservative.This antibody is purified through a protein A column.

Protein Information
Name	CCN2 (HGNC:2500)
Function	Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Is involved in the stimulation of osteoblast differentiation and has a critical role in osteogenesis (PubMed:39414788). Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor- induced DNA synthesis.
Cellular Location	Secreted, extracellular space, extracellular matrix {ECO:0000250\|UniProtKB:P29268}. Secreted
Tissue Location	Expressed in bone marrow and thymic cells. Also expressed one of two Wilms tumors tested.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.