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BLBP Recombinant Mouse mAb

Paraformaldehyde-fixed, paraffin embedded Rat cerebellum;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BLBP Monoclonal Antibody, Unconjugated (AP94810) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BLBP Monoclonal Antibody, Unconjugated (AP94810) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
(Negative control)Paraformaldehyde-fixed, paraffin embedded Rat kidney;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BLBP Monoclonal Antibody, Unconjugated (AP94810) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BLBP Monoclonal Antibody, Unconjugated (AP94810) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human glioma;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BLBP Monoclonal Antibody, Unconjugated (AP94810) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Brain;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BLBP Monoclonal Antibody, Unconjugated (AP94810) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
(Negative control)Paraformaldehyde-fixed, paraffin embedded Human kidney;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BLBP Monoclonal Antibody, Unconjugated (AP94810) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF
Primary Accession	P51880
Reactivity	Mouse
Host	Rabbit
Clonality	Recombinant
Calculated MW	14893 Da
Physical State	Liquid
Immunogen	Recombinant Mouse BLBP protein
Isotype	IgG1
Purity	Affinity Purification
Buffer	PBS with 100 µg/ml BSA,0.15% ProClin300 and 50% glycerol.
SUBCELLULAR LOCATION	Cytoplasm.
SIMILARITY	Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
SUBUNIT	Monomer.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	The protein encoded by this gene is a brain fatty acid binding protein. Fatty acid binding proteins (FABPs) are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. FABPs are thought to play roles in fatty acid uptake, transport, and metabolism. And is important in brain development.

Additional Information
Gene ID	12140
Other Names	Fatty acid-binding protein, brain, Brain lipid-binding protein, BLBP, Brain-type fatty acid-binding protein, B-FABP, Fatty acid-binding protein 7, Fabp7, Blbp
Target/Specificity	Expressed in brain and other neural tissues.
Dilution	WB=1: 500-1: 2000,IHC-P=1: 100-1: 500,IF=1: 100-1: 500,IHC-F=1: 100-1: 500
Format	0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Protein Information
Name	Fabp7
Synonyms	Blbp
Function	B-FABP could be involved in the transport of a so far unknown hydrophobic ligand with potential morphogenic activity during CNS development. It is required for the establishment of the radial glial fiber system in developing brain, a system that is necessary for the migration of immature neurons to establish cortical layers.
Cellular Location	Cytoplasm.
Tissue Location	Expressed in brain and other neural tissues.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol