NEFM Recombinant Mouse mAb
NEFM Recombinant Mouse mAb
- 产品详情
- 实验流程
- 背景知识
Application ![]()
| WB, IHC-P, IHC-F, IF |
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Reactivity | Mouse |
Host | Rabbit |
Clonality | Recombinant |
Calculated MW | 96 KDa |
Physical State | Liquid |
Immunogen | Recombinant Mouse NF-M protein |
Isotype | IgG1 |
Purity | Affinity Purification |
Buffer | PBS with 100 µg/ml BSA,0.15% ProClin300 and 50% glycerol. |
SIMILARITY | Belongs to the intermediate filament family. |
Post-translational modifications | There are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function.Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization. |
Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
Background Descriptions | Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber.May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks. |
Dilution | WB=1: 500-1: 2000,IHC-P=1: 500-1: 1000,IF=1: 500-1: 1000,IHC-F=1: 500-1: 1000 |
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Format | 0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce |
Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
Research Areas
For Research Use Only. Not For Use In Diagnostic Procedures.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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