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Cyclin G Rabbit pAb

Cyclin G Rabbit pAb

     
  • 14 - Cyclin G Rabbit pAb AP94788
    Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Cyclin G) Polyclonal Antibody, Unconjugated (AP94788) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
  • 14 - Cyclin G Rabbit pAb AP94788
    Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Cyclin G) Polyclonal Antibody, Unconjugated (AP94788) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
  • 14 - Cyclin G Rabbit pAb AP94788
    Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Cyclin G) Polyclonal Antibody, Unconjugated (AP94788) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
  • 14 - Cyclin G Rabbit pAb AP94788
    Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Cyclin G) Polyclonal Antibody, Unconjugated (AP94788) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
  • 1 - Cyclin G Rabbit pAb AP94788
    Sample: Lane 1: Human HepG2 cell lysates Lane 2: Human 293T cell lysates Lane 3: Human U87MG cell lysates Primary: Anti-Cyclin G (AP94788) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kDa Observed band size: 35 kDa
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, IHC-F, IF
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW 34 KDa
Physical State Liquid
Immunogen KLH conjugated synthetic peptide derived from mouse Cyclin G
Epitope Specificity 251-344/344
Isotype IgG
Purity affinity purified by Protein A
Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION Nucleus. DNA replication foci after DNA damage.
SIMILARITY Belongs to the cyclin family. Cyclin G subfamily.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions Cyclin G contains a typical N terminal cyclin box and a carboxy terminal domain sequence homologous to the tyrosine phosphorylation site of the epidermal growth factor receptor. Cyclin G2 shares 53% amino acid sequence identity with cyclin G1. Peak expression of cyclin G2 is seen in late S phase, as opposed to cyclin G1 expression, which is constitutive.
Additional Information
Target/Specificity High levels in skeletal muscle, ovary, kidney and colon.
Dilution IHC-P=1:100-500,WB=1:500-2000,IHC-F=1:100-500,IF=1:100-500
Format0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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