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SARS-CoV-2 (2019-nCoV) Spike RBD Mouse mAb

SARS-CoV-2 (2019-nCoV) Spike RBD Mouse mAb

     
  • 1 - SARS-CoV-2 (2019-nCoV) Spike RBD Mouse mAb AP94782
    Sample: Lane 1: SARS-CoV-2 Spike RBD Protein (His-Avi,HEK293) at 500ng Lane 2: SARS-CoV-2 S1 Protein (His-Avi,HEK293) at 500ng Primary: Mouse Anti-SARS-CoV-2 Spike RBD Protein Antibody at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 27/78kD Observed band size: kD
  • 1 - SARS-CoV-2 (2019-nCoV) Spike RBD Mouse mAb AP94782
    Sample: Lane 1: SARS-CoV-2 Spike RBD Protein(WT) at 500ng Lane 2: SARS-CoV-2 Spike S1 Protein(E484Q, L452R, D614G,P681R) at 500ng Lane 3: SARS-CoV-2 Spike S1 Protein (D80A, D215G, del241/243, K417N, E484K, N501Y, D614G) at 500ng Lane 4: SARS-CoV-2 Spike S1 Protein (L18F, T20N, P26S, D138Y, R190S,K417T,E484K,N501Y,D614G,H655Y) at 500ng Lane 5: SARS-CoV-2 Spike S1 Protein (WT) at 500ng Primary: Anti- SARS-CoV-2 Spike S1 Protein at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 27kDa /77.2 kDa Observed band size: 35kDa/114 kDa
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Host Rabbit
Clonality Monoclonal
Calculated MW 140 KDa
Physical State Liquid
Immunogen Recombinant SARS-CoV-2 Spike S1 Protein
Epitope Specificity 14-685/1213
Purity affinity purified by Protein A
Buffer 0.01M PBS (pH7.4).
SUBCELLULAR LOCATION Virion membrane ; Single-pass type I membrane protein ; Host endoplasmic reticulum-Golgi intermediate compartment membrane ; Single-pass type I membrane protein ; Host cell membrane ; Single-pass type I membrane protein ;Note: Accumulates in the endoplasmic reticulum-Golgi intermediate compartment, where it participates in virus particle assembly. Colocalizes with S in the host endoplasmic reticulum-Golgi intermediate compartment. Some S oligomers are transported to the host plasma membrane, where they may mediate cell-cell fusion.
SUBUNIT Spike glycoprotein: Homotrimer; each monomer consists of a S1 and a S2 subunit (PubMed:32155444, PubMed:32075877). The resulting peplomers protrude from the virus surface as spikes (By similarity). Interacts with the accessory proteins 3a and 7a.Spike protein S1: Binds to human ACE2.
Post-translational modifications The cytoplasmic Cys-rich domain is palmitoylated. Spike glycoprotein is digested within host endosomes.Specific enzymatic cleavages in vivo yield mature proteins. The precurssor is processed into S1 and S2 by host cell furin or another cellular protease to yield the mature S1 and S2 proteins (PubMed:32155444). Additionally, a second cleavage leads to the release of a fusion peptide after viral attachment to host cell receptor (By similarity). The presence of a furin polybasic cleavage site sets SARS-CoV-2 S apart from SARS-CoV S that possesses a monobasic S1/S2 cleavage site processed upon entry of target cells (PubMed:32155444).Highly decorated by heterogeneous N-linked glycans protruding from the trimer surface.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions The SARS-CoV-2 spike (S) protein is the target of vaccine design efforts to end the COVID-19 pandemic. Despite a low mutation rate, isolates with the D614G substitution in the S protein appeared early during the pandemic, and are now the dominant form worldwide. Here, we analyze the D614G mutation in the context of a soluble S ectodomain construct.
Additional Information
Target/Specificity The cytoplasmic Cys-rich domain is palmitoylated. Spike glycoprotein is digested within host endosomes.Specific enzymatic cleavages in vivo yield mature proteins. The precurssor is processed into S1 and S2 by host cell furin or another cellular protease to yield the mature S1 and S2 proteins (PubMed:32155444). Additionally, a second cleavage leads to the release of a fusion peptide after viral attachment to host cell receptor (By similarity). The presence of a furin polybasic cleavage site sets SARS-CoV-2 S apart from SARS-CoV S that possesses a monobasic S1/S2 cleavage site processed upon entry of target cells (PubMed:32155444).Highly decorated by heterogeneous N-linked glycans protruding from the trimer surface.
Dilution WB=1:500-2000
Format0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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