Slco1a1 Rabbit pAb

Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-OATP-1/Slco1a1 Polyclonal Antibody, Unconjugated(AP94652) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti-Slco1a1 (AP94652) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 74 kD Observed band size: 73 kD

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF
Primary Accession	P46720
Reactivity	Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	74178 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from rat Slco1a1
Epitope Specificity	2-90/670
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Basolateral cell membrane
SIMILARITY	Belongs to the organo anion transporter (TC 2.A.60) family. [SIMILARITY] Contains 1 Kazal-like domain.
SUBUNIT	Binds to PDZK1. Interaction with PDZK1 is required for expression on hepatocyte surface.
Post-translational modifications	Glycosylated.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	mediates the transport of sulfated, amidated bile acid, sulfolithocholyltaurine, into rat hepatocytes

Additional Information
Gene ID	50572
Other Names	Solute carrier organic anion transporter family member 1A1, Organic anion-transporting polypeptide 1, OATP-1, Sodium-independent organic anion transporter 1, Solute carrier family 21 member 1, Slco1a1 {ECO:0000312\|RGD:3700}
Target/Specificity	Highly expressed in liver and kidney, and at lower levels in brain, lung, skeletal muscle and proximal colon.
Dilution	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
Format	0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Protein Information
Name	Slco1a1 {ECO:0000312\|RGD:3700}
Function	Mediates the Na(+)-independent transport of organic anions such as steroid sulfate conjugates (dehydroepiandrosterone sulfate (DHEAS), 17-beta-glucuronosyl estradiol, estrone-3-sulfate), conjugated (taurocholate) and unconjugated (cholate) bile acids, prostaglandin E2 (PGE2) and L-thyroxine T4 (PubMed:19129463). Also capable of transporting sulfobromophthalein (BSP), ouabain and gadoxetate (By similarity). Hydrogencarbonate/HCO3(-) acts as the probable counteranion that exchanges for organic anions (PubMed:19129463). Shows a pH-sensitive substrate specificity which may be ascribed to the protonation state of the binding site and leads to a stimulation of substrate transport in an acidic microenvironment (PubMed:19129463).
Cellular Location	Basolateral cell membrane; Multi-pass membrane protein
Tissue Location	Highly expressed in liver and kidney, and at lower levels in brain, lung, skeletal muscle and proximal colon

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.