NR1D1 Rabbit pAb

Sample: BRL-3A (Rat) Cell Lysate at 40 ug Primary: Anti- NR1D1 (AP94534) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 67 kD Observed band size: 67 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NR1D1) Polyclonal Antibody, Unconjugated (AP94534) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Tissue/cell: human laryngeal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-NR1D1 Polyclonal Antibody, Unconjugated(AP94534) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-NR1D1/REV-ERB alpha Polyclonal Antibody, Unconjugated(AP94534) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control（black line）:Hela. Primary Antibody (green line): Rabbit Anti-NR1D1 antibody (AP94534) Dilution:1ug/Test; Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Isotype control（orange line）: Normal Rabbit IgG Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

产品详情
实验流程
背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Calculated MW	67 KDa
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human NR1D1
Epitope Specificity	551-614/614
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Nucleus (Potential).
SIMILARITY	Belongs to the nuclear hormone receptor family. NR1 subfamily.Contains 1 nuclear receptor DNA-binding domain.
SUBUNIT	Interacts with C1D and NR2E3. Interacts with SP1.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	NR1D1, a NR1 Thyroid Hormone-Like Receptor, is encoded by the same genomic locus as, but transcribed from the opposite strand of, Thyroid Hormone Receptor Alpha (TR Alpha). NR1D1 is a target of Nuclear Receptor ROR Alpha and a transcription regulator that has been shown to affect myocyte differentiation, adipogenesis, and lipoprotein metabolism. Mice lacking NR1D1 show abnormal postnatal cerebellar development. NR1D1 expression has been documented in human skeletal muscle and a variety of mouse and rat tissues. ESTs have been isolated from human tissue libraries, including cancerous adrenal, blood, brain, breast, colon, duodenum, fetus, head/neck, kidney, lung, skeletal muscle, skin, synovium, uterus, normal brain, breast, colon, eye, heart, pancreas, pituitary, prostate, skeletal muscle, skin, testis and thyroid.

Additional Information
Target/Specificity	Expressed in all tissues and cell lines examined. Expressed at high levels in some squamous carcinoma cell lines.
Dilution	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/Test
Format	0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol