HNRNPF Recombinant Mouse mAb

Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:5000 Primary ab incubation condition: room temperature 2h Secondary ab: Goat Anti-Mouse IgG H&L (HRP) Lysate: 293, A20, BRL, Daudi Protein loading quantity: 20 µg Exposure time: 60 s Predicted MW: 46 kDa Observed MW: 46 kDa
Cell line: HepG2 Fixative: 4% Paraformaldehyde Permeabilization: 0.1% TritonX-100 Primary ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary ab: Goat Anti-Mouse IgG Nuclear counter stain: DAPI (Blue) Counter stain: Tubulin (Red) Comment: Color green is the positive signal for PTM-6026

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF, ICC
Host	Rabbit
Clonality	Recombinant
Physical State	Liquid
Isotype	IgG1, Kappa
Purity	affinity purified by Protein G
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Nucleus, nucleoplasm.
SIMILARITY	Contains 3 RRM (RNA recognition motif) domains.
SUBUNIT	Identified in the spliceosome C complex. Interacts with AGO1, AGO2, TBP and TXNL4/DIM1.
Post-translational modifications	Sumoylated.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	hnRNP F is part of the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). These are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA) and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. hnRNP F binds to RNAs which have guanosine-rich sequences.

Additional Information
Target/Specificity	Expressed ubiquitously.
Dilution	WB=1:1000-1:5000,ICC/IF=1:50
Format	0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.