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CD89 Recombinant Mouse mAb

CD89 Recombinant Mouse mAb

     
  • 1 - CD89 Recombinant Mouse mAb AP94315
    Specimen: PBMC Fixative: Unfixed Permeabilization: None Primary Ab dilution: 1:100 Secondary Ab: Goat anti Mouse IgG Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line). Isotype control: Mouse monoclonal IgG1 (Blue line). Comment: Line red is the positive signal for AP94315
  • 1 - CD89 Recombinant Mouse mAb AP94315
    Cell line: Jurkat Fixative: 4% Paraformaldehyde Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary Ab: Goat Anti-Mouse IgG Nuclear counter stain: DAPI (Blue) Comment: Color green is the positive signal for AP94315
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IF, ICC
Host Rabbit
Clonality Recombinant
Physical State Liquid
Isotype IgG1, Kappa
Purity affinity purified by Protein G
Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION Isoform A.1: Cell membrane; Single-pass type I membrane protein. Isoform A.2: Cell membrane; Single-pass type I membrane protein.Isoform A.3: Cell membrane; Single-pass type I membrane protein.Isoform B: Secreted.Isoform B-delta-S2: Secreted.
SIMILARITY Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
SUBUNIT Associates with the Fc epsilon RI gamma 2 receptor inducing tyrosine phosphorylation of gamma 2.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions Binds to the Fc region of immunoglobulins alpha. Mediates several functions including cytokine production. Tissue specificity; Isoform A.1, isoform A.2 and isoform A.3 are differentially expressed between blood and mucosal myeloid cells. Isoform A.1, isoform A.2 and isoform A.3 are expressed in monocytes. Isoform A.1 and isoform A.2 are expressed in alveolar macrophages; however only one isoform is expressed at alveolar macrophages surfaces.
Additional Information
Target/Specificity Isoform A.1, isoform A.2 and isoform A.3 are differentially expressed between blood and mucosal myeloid cells. Isoform A.1, isoform A.2 and isoform A.3 are expressed in monocytes. Isoform A.1 and isoform A.2 are expressed in alveolar macrophages; however only one isoform is expressed at alveolar macrophages surfaces.
Dilution ICC/IF=1:50,Flow-Cyt=1:50-1:100
Format0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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