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RRM2 Recombinant Mouse mAb

RRM2 Recombinant Mouse mAb

     
  • 1 - RRM2 Recombinant Mouse mAb AP94304
    Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:3000 Primary ab incubation condition: 4°C overnight Secondary ab: Goat Anti-Mouse IgG H&L (HRP) Lysate:1: HepG2, 2: 293T Protein loading quantity: 20 µg Exposure time: 30 s Predicted MW: 45 kDa Observed MW: 45 kDa
  • 1 - RRM2 Recombinant Mouse mAb AP94304
    Cell line: HepG2 Fixative: 100% Ice-cold methanol Permeabilization: 0.1% TritonX-100 Primary ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary ab: Goat Anti-Mouse IgG Nuclear counter stain: DAPI (Blue) Comment: Color green is the positive signal for AP94304
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, ICC
Host Rabbit
Clonality Recombinant
Physical State Liquid
Isotype IgG1, Kappa
Purity affinity purified by Protein G
Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION Cytoplasm.
SIMILARITY Belongs to the ribonucleoside diphosphate reductase small chain family.
SUBUNIT Heterodimer of a large and a small subunit.
Post-translational modifications Phosphorylation on Ser-20 relieves the inhibitory effect on Wnt signaling.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions Ribonucleotide reductase is essential for the production and maintenance of the level of deoxyribonucleoside triphosphates (dNTPs) required for DNA synthesis. It is an enzymatic complex consisting of two nonidentical subunits, R1 and R2, which are inactive separately. R2, the smaller subunit, is localized to the cytoplasm. R2 is the limiting factor of the catalytic activity of the ribonucleotide reductase enzymatic complex. R2 expression is strictly correlated to the S-phase of the cell cycle, whereas R1 remains constant throughout all phases of the cell cycle. While R2 seems to be involved solely in the maintenance of dNTPs for DNA replication, a similar protein, p53R2, has been shown to be responsible for the production of dNTPs in response to DNA damage. Function : Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling.
Additional Information
Dilution WB=1:1000-1:3000,ICC/IF=1:50
Format0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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