癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
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GAPDH (Yeast) Rabbit pAb, Loading Control
GAPDH (Yeast) Rabbit pAb, Loading Control
- 产品详情
- 实验流程
- 背景知识
Application 
| WB |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 38 KDa |
| Physical State | Liquid |
| Immunogen | Recombinant Yeast GAPDH. |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SUBCELLULAR LOCATION | Cytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Membrane. Note=Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal. Postnuclear and Perinuclear regions. |
| SIMILARITY | Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. |
| SUBUNIT | Homotetramer (PubMed:16239728, PubMed:16510976).Interacts with TPPP; the interaction is direct (By similarity).Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation (By similarity).Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation (By similarity).Interacts with CHP1; the interaction increases the binding of CHP1 with microtubules (By similarity).Associates with microtubules (By similarity).Interacts with EIF1AD, USP25, PRKCI and WARS1 (PubMed:11724794, PubMed:16501887, PubMed:15628863, PubMed:20644585).Interacts with phosphorylated RPL13A; inhibited by oxidatively-modified low-densitity lipoprotein (LDL(ox)) (PubMed:22771119).Component of the GAIT complex (PubMed:15479637).Interacts with FKBP6; leading to inhibit GAPDH catalytic activity (PubMed:19001379).Interacts with TRAF2, promoting TRAF2 ubiquitination (PubMed:23332158).Interacts with TRAF3, promoting TRAF3 ubiquitination (PubMed:27387501). |
| Post-translational modifications | #Acetylation |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | oading Control Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 36kDa subunits. |
| Dilution | WB=1:1000-5000 |
|---|---|
| Format | 0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
Research Areas
For Research Use Only. Not For Use In Diagnostic Procedures.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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