human CD73 Mouse mAb

Blank control:A375. Primary Antibody (green line): Mouse Anti-human CD73 antibody (AP94187) Dilution: 1ug/Test; Secondary Antibody (white blue line) : Goat anti-Mouse IgG-AF488 Dilution: 0.5ug/Test. Isotype control（orange line）：Normal Mouse IgG Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Product Information
Host	Rabbit
Clonality	Monoclonal
Calculated MW	63 KDa
Physical State	Liquid
Isotype	Mouse IgG1, k
Purity	affinity purified by Protein G
Buffer	0.01M TBS (pH7.4).
SUBCELLULAR LOCATION	Cell membrane.
SIMILARITY	Belongs to the 5'-nucleotidase family.
DISEASE	Defects in NT5E are the cause of calcification of joints and arteries (CAJA). A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]

Additional Information
Dilution	Flow-Cyt=1ug/Test
Format	0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.