CD74 Rabbit pAb

Measured by its binding ability in a indirect ELISA. Immobilized Mouse CD74 protein, His Tag (Cat. bs-41374P) at 2 µg/mL (100 µL/well) can bind Rabbit Anti-Mouse CD74 Antibody, the EC50 is 669.6 ng/mL.
Paraformaldehyde-fixed, paraffin embedded (mouse thymus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD74) Polyclonal Antibody, Unconjugated (AP94149) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse lymphoid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD74) Polyclonal Antibody, Unconjugated (AP94149) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD74) Polyclonal Antibody, Unconjugated (AP94149) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, IHC-F, IF, E
Primary Accession	P04441
Reactivity	Mouse
Host	Rabbit
Clonality	Polyclonal
Calculated MW	31557 Da

Additional Information
Gene ID	16149
Other Names	H-2 class II histocompatibility antigen gamma chain, Ia antigen-associated invariant chain, Ii, Ii chain, MHC class II-associated invariant chain, CD74, Class-II-associated invariant chain peptide, Cd74 {ECO:0000312\|MGI:MGI:96534}
Dilution	IHC-P=1:400-800,IHC-F=1:400-800,IF=1:100-500,ELISA=1:5000-10000
Format	0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Protein Information
Name	Cd74 {ECO:0000312\|MGI:MGI:96534}
Function	Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to compartments where peptide loading of class II takes place. Enhance also the stimulation of T-cell responses through interaction with CD44.
Cellular Location	[Isoform Long]: Late endosome. Lysosome
Tissue Location	[Isoform Long]: Expressed in thymus and lymph noodes (PubMed:8977190). Expressed by antigen-presenting cells (APCs) (PubMed:11483509, PubMed:12417635).

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol