UBE2Q1 Rabbit pAb

cells:mouse splenocyte Concentration: 2 µg/10^6 cells, 4℃ incubate 30min.
Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-UBE2Q1 antibody (AP94048) Dilution: 2 µg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1 µg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Product Information
Primary Accession	Q7Z7E8.1
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Calculated MW	46 KDa
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human UBE2Q1
Epitope Specificity	321-422/422
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Additional Information
Dilution	Flow-Cyt=2 µg /Test
Format	0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.