TTLL12 (13V7) Mouse Monoclonal antibody

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TTLL12 antibody (AP93880), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TTLL12 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TTLL12. Positive lysates (100ug) and (20ug) can be purchased separately from biodragon.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB
Primary Accession	Q14166
Reactivity	Rat, Human, Mouse
Clonality	Monoclonal
Calculated MW	74404 Da

Additional Information
Gene ID	23170
Other Names	Tubulin--tyrosine ligase-like protein 12, Inactive tubulin--tyrosine ligase-like protein 12, TTLL12, KIAA0153
Dilution	WB~~1:1000
Storage Conditions	-20℃

Protein Information
Name	TTLL12
Synonyms	KIAA0153
Function	Negatively regulates post-translational modifications of tubulin, including detyrosination of the C-terminus and polyglutamylation of glutamate residues (PubMed:20162578, PubMed:23251473). Also, indirectly promotes histone H4 trimethylation at 'Lys-20' (H4K20me3) (PubMed:23251473). Probably by controlling tubulin and/or histone H4 post-translational modifications, plays a role in mitosis and in maintaining chromosome number stability (PubMed:20162578, PubMed:23251473). During RNA virus-mediated infection, acts as a negative regulator of the RIG-I pathway by preventing MAVS binding to TBK1 and IKBKE (PubMed:28011935).
Cellular Location	Cytoplasm. Midbody Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Nucleus Note=Predominantly localizes in the cytoplasm (PubMed:28011935) Partially colocalizes with vimentin in prostate cancer cells (PubMed:20162578).
Tissue Location	Expressed in the basal layer of prostate and endothelial cells. Increased expression in prostatic intraepithelial neoplasia and metastatic lesions.