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PPA2 (6W7) Mouse Monoclonal antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, AP93864) (1:150)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, AP93864) (1:150)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, AP93864) (1:150)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, AP93864) (1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPA2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPA2 (Cat# AP93864)(1:2000). Positive lysates (100ug) and (20ug) can be purchased separately from biodragon.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	Q9H2U2
Reactivity	Human
Clonality	Monoclonal
Calculated MW	37920 Da

Additional Information
Gene ID	27068
Other Names	Inorganic pyrophosphatase 2, mitochondrial, 3.6.1.1, Pyrophosphatase SID6-306, Pyrophosphate phospho-hydrolase 2, PPase 2, PPA2
Dilution	WB~~1:1000 IHC~~1:100~500
Storage Conditions	-20℃

Protein Information
Name	PPA2
Function	Hydrolyzes inorganic pyrophosphate (PubMed:27523597). This activity is essential for correct regulation of mitochondrial membrane potential, and mitochondrial organization and function (PubMed:27523598).
Cellular Location	Mitochondrion.
Tissue Location	Detected in brain, gastric carcinoma, lung, ovary, skeletal muscle, umbilical cord blood and a cell line derived from kidney proximal tubule epithelium.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

The protein encoded by this gene is localized to the mitochondrion, is highly similar to members of the inorganic pyrophosphatase (PPase) family, and contains the signature sequence essential for the catalytic activity of PPase. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

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¥ 1,500.00

Cat# AP93864

Size:

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100 µl

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¥ 1,500.00

¥ 2,600.00

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PPA2 (6W7) Mouse Monoclonal antibody

PPA2 (6W7) Mouse Monoclonal antibody

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