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Rab11b (6D13) Rat Monoclonal Antibody

IHC-P analysis of mouse brain tissue by Rab11b antibody (AP93647). IHC-P was performed using sections of the formalin-fixed paraffin-embedded mouse brain tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2for 30 min at room temperature. The sections were then incubated with Rab11b antibody (AP93647) at 20 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG (which cross reacts with rat IgG) was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control. Result: Neurons are positively stained at the cytoplasm.

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实验流程

Product Information
Primary Accession	P46638
Clonality	Monoclonal
Calculated MW	24489 Da

Additional Information
Gene ID	19326
Other Names	Ras-related protein Rab-11B, 3.6.5.2, Rab11b
Storage Conditions	-20℃

Protein Information
Name	Rab11b {ECO:0000312\|MGI:MGI:99425}
Function	The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. The small Rab GTPase RAB11B plays a role in endocytic recycling, regulating apical recycling of several transmembrane proteins including cystic fibrosis transmembrane conductance regulator/CFTR, epithelial sodium channel/ENaC, potassium voltage-gated channel, and voltage-dependent L-type calcium channel. May also regulate constitutive and regulated secretion, like insulin granule exocytosis. Required for melanosome transport and release from melanocytes. Also regulates V-ATPase intracellular transport in response to extracellular acidosis. Promotes Rabin8/RAB3IP preciliary vesicular trafficking to mother centriole by forming a ciliary targeting complex containing Rab11, ASAP1, Rabin8/RAB3IP, RAB11FIP3 and ARF4, thereby regulating ciliogenesis initiation. On the contrary, upon LPAR1 receptor signaling pathway activation, interaction with phosphorylated WDR44 prevents Rab11-RAB3IP-RAB11FIP3 complex formation and cilia growth (By similarity).
Cellular Location	Recycling endosome membrane; Lipid-anchor; Cytoplasmic side. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane {ECO:0000250\|UniProtKB:O35509}; Lipid-anchor {ECO:0000250\|UniProtKB:O35509}; Cytoplasmic side {ECO:0000250\|UniProtKB:O35509}. Cytoplasmic vesicle, phagosome membrane {ECO:0000250\|UniProtKB:Q15907}; Lipid-anchor {ECO:0000250\|UniProtKB:Q15907}; Cytoplasmic side {ECO:0000250\|UniProtKB:Q15907}. Cytoplasmic vesicle {ECO:0000250\|UniProtKB:Q15907}. Note=Colocalizes with RAB11AFIP1 on punctate vesicles. {ECO:0000250\|UniProtKB:Q15907}
Tissue Location	Abundantly expressed in brain, heart and testis. Also detected in kidney and pancreatic islets

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol