Rab6b (14D18) Rat Monoclonal Antibody

Various protein samples were run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. AP93632 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-rat IgG was used as the secondary antibody. Rab6b band was visualized using ECL Substrate. Lane 1: 15 µg of SH-SY5Y lysate Lane 2: 15 µg of mouse brain tissue lysate Lane 3: 15 µg of rat brain tissue lysate Result: AP93632 can detect Rab6b by Western blotting.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB
Primary Accession	P61294
Reactivity	Rat, Mouse
Clonality	Monoclonal
Calculated MW	23462 Da

Additional Information
Gene ID	270192
Other Names	Ras-related protein Rab-6B, 3.6.5.2, Rab6b, D9Bwg0185e
Dilution	WB~~1:1000
Storage Conditions	-20℃

Protein Information
Name	Rab6b {ECO:0000312\|MGI:MGI:107283}
Synonyms	D9Bwg0185e
Function	The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between active GTP-bound and inactive GDP-bound states. In their active state, drive transport of vesicular carriers from donor organelles to acceptor organelles to regulate the membrane traffic that maintains organelle identity and morphology. Recruits VPS13B to the Golgi membrane. Regulates the compacted morphology of the Golgi. Seems to have a role in retrograde membrane traffic at the level of the Golgi complex. May function in retrograde transport in neuronal cells. Plays a role in neuron projection development.
Cellular Location	Golgi apparatus membrane {ECO:0000250\|UniProtKB:Q9NRW1}; Lipid-anchor. Endoplasmic reticulum-Golgi intermediate compartment {ECO:0000250\|UniProtKB:Q9NRW1}. Cytoplasmic vesicle {ECO:0000250\|UniProtKB:Q9NRW1}. Note=Colocalizes with BICD1 at vesicular structures that align along microtubules {ECO:0000250\|UniProtKB:Q9NRW1}

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.