> 首页 > 产品 > 一抗 > 代谢 > GUSB Antibody (Center)

GUSB Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

标注"皇冠"的产品，是满足高质量标准的产品。
了解更多...

Immunohistochemical analysis of AP9348C on paraffin-embedded Human colon carcinoma tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Immunohistochemical analysis of AP9348C on paraffin-embedded Human liver tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
All lanes : Anti-GUSB Antibody (Center) at 1:4000 dilution Lane 1: HT-29 whole cell lysate Lane 2: WiDr whole cell lysate Lane 3: K562 whole cell lysate Lane 4: A2058 whole cell lysate Lane 5: HL-60 whole cell lysate Lane 6: Mouse liver tissue lysate Lane 7: Mouse lung tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 75 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
GUSB Antibody (Center) (Cat. #AP9348c) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

产品详情
实验流程
背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, FC, WB, E
Primary Accession	P08236
Other Accession	Q4FAT7
Reactivity	Human, Mouse
Predicted	Pig
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	74732 Da
Antigen Region	335-362 aa

Additional Information
Gene ID	2990
Other Names	Beta-glucuronidase, Beta-G1, GUSB
Target/Specificity	This GUSB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 335-362 amino acids from the Central region of human GUSB.
Dilution	IHC-P~~1:100 FC~~1:10~50 WB~~1:4000 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	GUSB Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	GUSB
Function	Plays an important role in the degradation of dermatan and keratan sulfates.
Cellular Location	Lysosome.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

The GUSB gene encodes beta-glucuronidase (EC 3.2.1.31), a lysosomal hydrolase involved in the stepwise degradation of glucuronic acid-containing glycosaminoglycans (Shipley et al., 1993 [PubMed 7680524]). It is a tetrameric glycoprotein composed of identical subunits (Oshima et al., 1987 [PubMed 3468507]). The GUSB gene is mutated in mucopolysaccharidosis type VII (MPS7; MIM 253220).

REFERENCES

Tomatsu,S. Hum. Mutat. 30 (4), 511-519 (2009)
Romanowski,T. Med. Sci. Monit. 14 (7), BR147-BR152 (2008)
Gratz,M. Pharmacogenet. Genomics 15 (12), 875-881 (2005)

终于等到您。ABCEPTA（百远生物）抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息，您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问，联系：0512-88856768 tech-china@abcepta.com.

我要评价

¥ 1,250.00

Cat# AP9348c

Size:

50 µl

100 µl

200 µl

400 µl

Price:

¥ 1,250.00

¥ 2,050.00

¥ 2,960.00

¥ 4,550.00

Quantity:

reduce add

Availability: In Stock

询价

GUSB Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

BACKGROUND

REFERENCES

相关产品

多肽合成服务

多克隆抗体制备服务

单克隆抗体制备服务

抗体测序服务

蛋白表达服务

药物发现定制服务