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DLL3 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

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Immunohistochemical analysis of paraffin-embedded human brain tissue using AP9328B performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
All lanes : Anti-DLL3 Antibody (C-term) at 1:2000 dilution Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 65 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Confocal immunofluorescent analysis of DLL3 Antibody (C-term)(Cat#AP9328b) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P-Leica, IF, WB, E
Primary Accession	Q9NYJ7
Reactivity	Human, Rat, Mouse
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	64618 Da
Antigen Region	519-548 aa

Additional Information
Gene ID	10683
Other Names	Delta-like protein 3, Drosophila Delta homolog 3, Delta3, DLL3
Target/Specificity	This DLL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 519-548 amino acids from the C-terminal region of human DLL3.
Dilution	IHC-P-Leica~~1:500 IF~~1:10~50 WB~~1:2000 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	DLL3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	DLL3
Function	Inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm (By similarity).
Cellular Location	Membrane; Single-pass type I membrane protein

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

DLL3 encodes a member of the delta protein ligand family. This family functions as Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain.

REFERENCES

Yerges,L.M. J. Bone Miner. Res. 24 (12), 2039-2049 (2009)
Heuss,S.F. Proc. Natl. Acad. Sci. U.S.A. 105 (32), 11212-11217 (2008)
Maisenbacher,M.K. Hum. Genet. 116 (5), 416-419 (2005)

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¥ 1,250.00

Cat# AP9328B

Size:

50 µl

100 µl

200 µl

400 µl

Price:

¥ 1,250.00

¥ 2,050.00

¥ 2,960.00

¥ 4,550.00

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Availability: In Stock

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DLL3 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

BACKGROUND

REFERENCES

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