K27-linkage Specific Ubiquitin Antibody

Rabbit mAb

Western blot analysis of K27-linkage Specific Ubiquitin expression in HeLa cell lysate.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF, ICC, IHF
Primary Accession	P0CG47/P0CG48
Reactivity	Rat, Human, Mouse
Clonality	Monoclonal
Other Names	FLJ25987; MGC8385; ubiquitin B; Ubiquitin; UBCEP1; UBCEP2; RPS27A
Isotype	Rabbit IgG
Host	Rabbit
Calculated MW	22 KDa

Additional Information
Dilution	WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200
Purification	Affinity-chromatography
Immunogen	A synthesized peptide derived from human K27-linkage Specific Ubiquitin
Description	Plays an important role in the ubiquitin-proteasome pathway. Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue.
Storage Condition and Buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.