BRAT1 Antibody

Rabbit mAb

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF, FC, ICC, IP
Primary Accession	Q6PJG6
Reactivity	Human
Clonality	Monoclonal
Other Names	BAAT1; brat1; C7orf27; RMFSL;
Isotype	Rabbit IgG
Host	Rabbit
Calculated MW	88119 Da

Additional Information
Dilution	WB 1:500~1:2000 ICC/IF 1:50~1:200 IP 1:50 FC 1:50
Purification	Affinity-chromatography
Immunogen	A synthesized peptide derived from human BRAT1
Description	Required for activation of ATM following ionizing radiation. May act by regulating dephosphorylation of ATM.
Storage Condition and Buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Protein Information
Name	BRAT1 {ECO:0000303\|PubMed:25657994, ECO:0000312\|HGNC:HGNC:21701}
Function	Component of a multiprotein complex required for the assembly of the RNA endonuclease module of the integrator complex (PubMed:39032489, PubMed:39032490). Associates with INTS9 and INTS11 in the cytoplasm and blocks the active site of INTS11 to inhibit the endonuclease activity of INTS11 before formation of the full integrator complex (PubMed:39032489, PubMed:39032490). Following dissociation of WDR73 of the complex, BRAT1 facilitates the nuclear import of the INTS9-INTS11 heterodimer (PubMed:39032489). In the nucleus, INTS4 is integrated to the INTS9-INTS11 heterodimer and BRAT1 is released from the mature RNA endonuclease module by inositol hexakisphosphate (InsP6) (PubMed:39032489). BRAT1 is also involved in DNA damage response; activates kinases ATM, SMC1A and PRKDC by modulating their phosphorylation status following ionizing radiation (IR) stress (PubMed:16452482, PubMed:22977523). Plays a role in regulating mitochondrial function and cell proliferation (PubMed:25070371). Required for protein stability of MTOR and MTOR-related proteins, and cell cycle progress by growth factors (PubMed:25657994).
Cellular Location	Nucleus. Cytoplasm Note=Present at double strand breaks (DSBs)following ionizing radiation treatment. The ubiquitinated form localizes in the nucleus in a NDFIP1- dependent manner.
Tissue Location	Ubiquitously expressed.