DDAH2 Antibody

Rabbit mAb

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IP
Primary Accession	O95865
Reactivity	Rat, Human, Mouse
Clonality	Monoclonal
Other Names	DDAH; DDAH II; DDAH2; DDAHII; Dimethylargininase 2; G6a;
Isotype	Rabbit IgG
Host	Rabbit
Calculated MW	29644 Da

Additional Information
Dilution	WB 1:500~1:2000 IP 1:50
Purification	Affinity-chromatography
Immunogen	A synthesized peptide derived from human DDAH2
Description	Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
Storage Condition and Buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Protein Information
Name	DDAH2 (HGNC:2716)
Synonyms	DDAH, G6A, NG30
Function	Putative hydrolase with unknown substrate (Probable). Does not hydrolyze N(G),N(G)-dimethyl-L-arginine (ADMA) which acts as an inhibitor of NOS (PubMed:21493890, PubMed:37296100). In endothelial cells, induces expression of vascular endothelial growth factor (VEGF) via phosphorylation of the transcription factor SP1 by PKA in a process that is independent of NO and NO synthase (By similarity). Similarly, enhances pancreatic insulin secretion through SP1-mediated transcriptional up-regulation of secretagogin/SCGN, an insulin vesicle docking protein (By similarity). Upon viral infection, relocates to mitochondria where it promotes mitochondrial fission through activation of DNM1L leading to the inhibition of innate response activation mediated by MAVS (PubMed:33850055).
Cellular Location	Cytoplasm. Mitochondrion Note=Translocates from cytosol to mitochondrion upon IL1B stimulation in chondrocytes
Tissue Location	Detected in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas, and at very low levels in brain