IQGAP1 Antibody

Rabbit mAb

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF, FC, ICC, IHF
Primary Accession	P46940
Reactivity	Human, Mouse
Clonality	Monoclonal
Other Names	Iqgap1; p195; SAR1;
Isotype	Rabbit IgG
Host	Rabbit
Calculated MW	189252 Da

Additional Information
Dilution	WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 FC 1:50
Purification	Affinity-chromatography
Immunogen	A synthesized peptide derived from human IQGAP1
Description	Binds to activated CDC42 but does not stimulate its GTPase activity. It associates with calmodulin. Could serve as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane.
Storage Condition and Buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Protein Information
Name	IQGAP1
Synonyms	KIAA0051
Function	Plays a crucial role in regulating the dynamics and assembly of the actin cytoskeleton. Recruited to the cell cortex by interaction with ILK which allows it to cooperate with its effector DIAPH1 to locally stabilize microtubules and allow stable insertion of caveolae into the plasma membrane (By similarity). Binds to activated CDC42 but does not stimulate its GTPase activity. Associates with calmodulin. May promote neurite outgrowth (PubMed:15695813). May play a possible role in cell cycle regulation by contributing to cell cycle progression after DNA replication arrest (PubMed:20883816).
Cellular Location	Cell membrane. Nucleus. Cytoplasm. Cytoplasm, cell cortex {ECO:0000250\|UniProtKB:Q9JKF1}. Apical cell membrane. Basolateral cell membrane {ECO:0000250\|UniProtKB:Q9JKF1}. Note=Subcellular distribution is regulated by the cell cycle, nuclear levels increase at G1/S phase (PubMed:20883816).
Tissue Location	Expressed in the placenta, lung, and kidney (PubMed:8051149). A lower level expression is seen in the heart, liver, skeletal muscle and pancreas (PubMed:8051149)