Phospho-POLR2A (S2) Antibody

Rabbit mAb

Western blot analysis of Phospho-POLR2A (S2) expression in (1) MCF7 cell lysate treated with Lambda Phosphatase; (2) MCF7 cell lysate.
Immunohistochemical analysis of paraffin-embedded human spleen, using Phospho-POLR2A (S2) Antibody.
Immunofluorescent analysis of MCF7 cells, using Phospho-POLR2A (S2) Antibody .

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF, FC, ICC, IP, IHF
Primary Accession	P24928
Reactivity	Rat, Human, Mouse
Clonality	Monoclonal
Other Names	POLR2A; POLR2; RNA polymerase II CTD repeat YSPTSPS;
Isotype	Rabbit IgG
Host	Rabbit
Calculated MW	270 KDa

Additional Information
Dilution	WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50 FC 1:50
Purification	Affinity-chromatography
Immunogen	A synthesized peptide derived from human POLR2A
Description	During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing.
Storage Condition and Buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.