癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
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PGK1 Antibody
Rabbit mAb
- 产品详情
- 实验流程
Application 
| WB, IF, FC, ICC |
|---|---|
| Primary Accession | P00558 |
| Reactivity | Rat, Human, Mouse |
| Clonality | Monoclonal |
| Other Names | MGC117307; MGC142128; MGC8947; MIG10; PGKA; PGK1; PRP2; |
| Isotype | Rabbit IgG |
| Host | Rabbit |
| Calculated MW | 44615 Da |
| Dilution | WB 1:500~1:2000 ICC/IF 1:50~1:200 FC 1:50 |
|---|---|
| Purification | Affinity-chromatography |
| Immunogen | A synthesized peptide derived from human PGK1 |
| Description | The PGK1 gene encodes phosphoglycerate kinase-1, also known as ATP:3-phosphoglycerate 1-phosphotransferase (EC 2.7.2.3), which catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. It Belongs to the phosphoglycerate kinase family and defects in PGK1 are the cause of phosphoglycerate kinase 1 deficiency (PGK1D). |
| Storage Condition and Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle. |
| Name | PGK1 |
|---|---|
| Synonyms | PGKA |
| Function | Catalyzes one of the two ATP producing reactions in the glycolytic pathway via the reversible conversion of 1,3- diphosphoglycerate to 3-phosphoglycerate (PubMed:30323285, PubMed:7391028). Both L- and D- forms of purine and pyrimidine nucleotides can be used as substrates, but the activity is much lower on pyrimidines (PubMed:18463139). In addition to its role as a glycolytic enzyme, it seems that PGK1 acts as a polymerase alpha cofactor protein (primer recognition protein) (PubMed:2324090). Acts as a protein kinase when localized to the mitochondrion where it phosphorylates pyruvate dehydrogenase kinase PDK1 to inhibit pyruvate dehydrogenase complex activity and suppress the formation of acetyl- coenzyme A from pyruvate, and consequently inhibit oxidative phosphorylation and promote glycolysis (PubMed:26942675, PubMed:36849569). May play a role in sperm motility (PubMed:26677959). |
| Cellular Location | Cytoplasm, cytosol. Mitochondrion matrix. Note=Hypoxic conditions promote mitochondrial targeting (PubMed:26942675). Targeted to the mitochondrion following phosphorylation by MAPK1/ERK2, cis-trans isomerization by PIN1, and binding to mitochondrial circRNA mcPGK1 (PubMed:36849569). |
| Tissue Location | Mainly expressed in spermatogonia. Localized on the principle piece in the sperm (at protein level). Expression significantly decreased in the testis of elderly men |
Research Areas
For Research Use Only. Not For Use In Diagnostic Procedures.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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¥ 1,500.00
Cat# AP90539
