Cyclin D1 Antibody

Rabbit mAb

Western blot analysis of Cyclin D1 expression in (1)MCF-7 cell lysates;(2) LnCaP cell lysates.
Immunohistochemical analysis of paraffin-embedded human bladder, using Cyclin D1 Antibody.
Immunofluorescent analysis of MCF-7 cells, using Cyclin D1 Antibody .
miR‐139‐5p reverses stemness maintenance and metastasis of colon cancer stem‐like cells by targeting E2‐2. -JOURNAL OF CELLULAR PHYSIOLOGY

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF, ICC, IP, IHF
Primary Accession	P24385
Reactivity	Rat, Human, Mouse
Clonality	Monoclonal
Other Names	CCND1;BCL1; D11S287E; PRAD1; U21B31; Cyclin D1;
Isotype	Rabbit IgG
Host	Rabbit
Calculated MW	34 KDa

Additional Information
Dilution	WB 1:1000~1:5000 IHC 1:50~1:200 ICC/IF 1:50~1:100 IP 1:50
Purification	Affinity-chromatography
Immunogen	A synthesized peptide derived from human Cyclin D1
Description	Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals.
Storage Condition and Buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.