注册 | 登录
点击这里给我发消息
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   重组兔单克隆抗体   >   重组兔单克隆抗体   >   p21 Antibody   

p21 Antibody

Rabbit mAb

     
  • 1 - p21 Antibody AP90151
    Western blot analysis of p21 in (1) MCF-7 cell lysate; (2) LnCaP cell lysate.
  • 2 - p21 Antibody AP90151
    Immunohistochemical analysis of paraffin-embedded human colon, using p21 Antibody.
  • 2 - p21 Antibody AP90151
    Immunofluorescent analysis of MCF7 cells, using p21 Antibody .
  • 1 - p21 Antibody AP90151
    Expression of estrogen receptors and androgen receptor and their clinical significance in gastric cancer. -Oncotarget
  • 产品详情
  • 实验流程
Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC, IF, ICC, IP, IHF
Primary Accession P38936
Reactivity Human
Clonality Monoclonal
Other Names CAP20; CDKN1; CIP1; MDA-6; P21; SDI1; WAF1; P21cip1;
Isotype Rabbit IgG
Host Rabbit
Calculated MW 18119 Da
Additional Information
Dilution WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50
Purification Affinity-chromatography
Immunogen A synthesized peptide derived from human p21
Description The tumor suppressor protein p21 Waf1/Cip1 acts as an inhibitor of cell cycle progression. It functions in stoichiometric relationships forming heterotrimeric complexes with cyclins and cyclin-dependent kinases. In association with CDK2 complexes, it serves to inhibit kinase activity and block progression through G1/S. However, p21 may also enhance assembly and activity in complexes of CDK4 or CDK6 and cyclin D.
Storage Condition and Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Protein Information
Name CDKN1A (HGNC:1784)
Function Plays an important role in controlling cell cycle progression and DNA damage-induced G2 arrest (PubMed:9106657). Involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Also involved in p53-independent DNA damage-induced G2 arrest mediated by CREB3L1 in astrocytes and osteoblasts (By similarity). Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding (PubMed:11595739). Negatively regulates the CDK4- and CDK6-driven phosphorylation of RB1 in keratinocytes, thereby resulting in the release of E2F1 and subsequent transcription of E2F1-driven G1/S phase promoting genes (By similarity).
Cellular Location Cytoplasm. Nucleus
Tissue Location Expressed in all adult tissues, with 5-fold lower levels observed in the brain
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

FeedBack

终于等到您。ABCEPTA(百远生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问,联系:0512-88856768 tech-china@abcepta.com.


我要评价