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FCGR2A Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - FCGR2A Antibody (C-term) AP8918b
    Western blot analysis of lysate from Jurkat cell line,using FCGR2A Antibody (C-term)(Cat. #AP8918b).AP8918b was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane.
  • 4 - FCGR2A Antibody (C-term) AP8918b
    FCGR2A Antibody (C-term) (Cat. #AP8918b) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, WB, E
Primary Accession P12318
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 35001 Da
Antigen Region 255-282 aa
Additional Information
Gene ID 2212
Other Names Low affinity immunoglobulin gamma Fc region receptor II-a, IgG Fc receptor II-a, CDw32, Fc-gamma RII-a, Fc-gamma-RIIa, FcRII-a, CD32, FCGR2A, CD32, FCG2, FCGR2A1, IGFR2
Target/Specificity This FCGR2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 255-282 amino acids from the C-terminal region of human FCGR2A.
Dilution FC~~1:10~50
WB~~1:1000
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFCGR2A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FCGR2A
Synonyms CD32, FCG2, FCGR2A1, IGFR2
Function Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens.
Cellular Location Cell membrane; Single-pass type I membrane protein
Tissue Location Found on monocytes, neutrophils and eosinophil platelets
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

FCGR2A is one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants.

REFERENCES

Staudt,A.et.al, Clin. Pharmacol. Ther. (2010) In press

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