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>   首页   >   产品   >   一抗   >   癌症   >   MAP2K5 Antibody (S149)   

MAP2K5 Antibody (S149)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - MAP2K5 Antibody (S149) AP7906f
    Western blot analysis of MAP2K5 (arrow) using rabbit polyclonal MAP2K5 Antibody (S149) (Cat.#AP7906f).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K5 gene (Lane 2) (Origene Technologies).
  • 14 - MAP2K5 Antibody (S149) AP7906f
    Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with MAP2K5 Antibody (S149) (Cat.#AP7906f), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q13163
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 50112 Da
Antigen Region 127-156 aa
Additional Information
Gene ID 5607
Other Names Dual specificity mitogen-activated protein kinase kinase 5, MAP kinase kinase 5, MAPKK 5, MAPK/ERK kinase 5, MEK 5, MAP2K5, MEK5, MKK5, PRKMK5
Target/Specificity This MAP2K5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 127-156 amino acids from human MAP2K5.
Dilution WB~~1:1000
IHC-P~~1:100~500
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMAP2K5 Antibody (S149) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MAP2K5
Synonyms MEK5, MKK5, PRKMK5
Function Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appears to play a critical role in protecting cells from stress-induced apoptosis, neuronal survival and cardiac development and angiogenesis. As part of the MAPK/ERK signaling pathway, acts as a negative regulator of apoptosis in cardiomyocytes via promotion of STUB1/CHIP-mediated ubiquitination and degradation of ICER-type isoforms of CREM (By similarity).
Tissue Location Expressed in many adult tissues. Abundant in heart and skeletal muscle
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

MAP2K5 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. It specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by MAP2K5 is involved in growth factor stimulated cell proliferation and muscle cell differentiation.

REFERENCES

Raviv,Z.,J. Cell. Sci. 117 (PT 9), 1773-1784 (2004)
Kato,Y.,EMBO J. 16 (23), 7054-7066 (1997)
English,J.M.,J. Biol. Chem. 274 (44), 31588-31592 (1999)

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