> 首页 > 产品 > 一抗 > 信号转导 > TBK1 Antibody (S172)

TBK1 Antibody (S172)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

标注"皇冠"的产品，是满足高质量标准的产品。
了解更多...

AP7887a staining TBK1 in human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
All lanes : Anti-TBK1 Antibody (S172) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HT-1080 whole cell lysate Lane 3: K562 whole cell lysate Lane 4: mouse testis lysate Lane 5: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size :83kDa Blocking/Dilution buffer: 5% NFDM/TBST.

产品详情
文献引用 : 1
实验流程
背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, E
Primary Accession	Q9UHD2
Other Accession	Q6DFJ6, Q9WUN2, NP_037386
Reactivity	Human, Mouse
Predicted	Mouse, Xenopus
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Antigen Region	150-181 aa

Additional Information
Other Names	Serine/threonine-protein kinase TBK1, NF-kappa-B-activating kinase, T2K, TANK-binding kinase 1, TBK1, NAK
Target/Specificity	This TBK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 150-181 amino acids from human TBK.
Dilution	WB~~1:2000 IHC-P~~1:100~500 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	TBK1 Antibody (S172) is for research use only and not for use in diagnostic or therapeutic procedures.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

The NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. TKB is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors. The protein can form a complex with the IKB protein TANK and TRAF2 and release the NFKB inhibition caused by TANK.

REFERENCES

Deng,W., J. Biol. Chem. 283 (51), 35590-35597 (2008)
Chessler,A.D., J. Immunol. 181 (11), 7917-7924 (2008)
Soulat,D., EMBO J. 27 (15), 2135-2146 (2008)

终于等到您。ABCEPTA（百远生物）抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息，您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问，联系：0512-88856768 tech-china@abcepta.com.

我要评价

¥ 699.00

Cat# AP7887a

Size:

25 ul

50 µl

100 µl

200 µl

400 µl

Price:

¥ 699.00

¥ 1,250.00

¥ 2,050.00

¥ 2,960.00

¥ 4,550.00

Quantity:

reduce add

Availability: In Stock

询价

TBK1 Antibody (S172)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

BACKGROUND

REFERENCES

相关产品

多肽合成服务

多克隆抗体制备服务

单克隆抗体制备服务

抗体测序服务

蛋白表达服务

药物发现定制服务