GARS Rabbit mAb

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P
Primary Accession	P41250
Reactivity	Rat, Human, Mouse
Host	Rabbit
Clonality	Monoclonal Antibody
Isotype	IgG
Conjugate	Unconjugated
Immunogen	A synthesized peptide derived from human GARS
Purification	Affinity Chromatography
Calculated MW	83166 Da

Additional Information
Gene ID	2617
Other Names	GARS1
Dilution	WB~~1/500-1/1000 IHC-P~~N/A
Format	Liquid in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Protein Information
Name	GARS1 (HGNC:4162)
Synonyms	GARS
Function	Catalyzes the ATP-dependent ligation of glycine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (Gly-AMP) (PubMed:17544401, PubMed:24898252, PubMed:28675565). Also produces diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs. Thereby, may play a special role in Ap4A homeostasis (PubMed:19710017).
Cellular Location	Cytoplasm. Cell projection, axon. Secreted {ECO:0000250\|UniProtKB:Q9CZD3}. Secreted, extracellular exosome {ECO:0000250\|UniProtKB:Q9CZD3}. Note=In transfected COS7 cells, not detected in mitochondria, nor in Golgi apparatus (PubMed:17035524) Secreted by motor neuron, possibly through the exosome pathway (By similarity). {ECO:0000250\|UniProtKB:Q9CZD3, ECO:0000269\|PubMed:17035524} [Isoform 2]: Cytoplasm. Cell projection, axon
Tissue Location	Widely expressed, including in brain and spinal cord. [Isoform 1]: Expressed in brain, spinal cord, muscle, heart, spleen and liver.