HisRS Polyclonal Antibody

Western Blot analysis of various cells using HisRS Polyclonal Antibody diluted at 1：2000

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IF
Primary Accession	P12081
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Calculated MW	57411 Da

Additional Information
Gene ID	3035
Other Names	HARS; HRS; Histidine--tRNA ligase; cytoplasmic; Histidyl-tRNA synthetase; HisRS
Dilution	WB~~Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. IHC-P~~N/A IF~~1:50~200
Format	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% (W/V) sodium azide.
Storage Conditions	-20℃

Protein Information
Name	HARS1 (HGNC:4816)
Synonyms	HARS, HRS
Function	Catalyzes the ATP-dependent ligation of histidine to the 3'- end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (His-AMP) (PubMed:29235198). Plays a role in axon guidance (PubMed:26072516).
Cellular Location	Cytoplasm {ECO:0000250\|UniProtKB:F1Q5D5}.
Tissue Location	Brain, heart, liver and kidney.