GK1 Polyclonal Antibody
- 产品详情
- 实验流程
- 背景知识
Application ![]()
| WB, IF |
---|---|
Primary Accession | P32189 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | 61245 Da |
Gene ID | 2710 |
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Other Names | GK; Glycerol kinase; GK; Glycerokinase; ATP:glycerol 3-phosphotransferase |
Dilution | WB~~Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. IF~~1:50~200 |
Format | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% (W/V) sodium azide. |
Storage Conditions | -20℃ |
Name | GK (HGNC:4289) |
---|---|
Function | Kinase that plays a key role in glycerol metabolism, catalyzing its phosphorylation to produce sn-glycerol 3-phosphate. Sn- glycerol 3-phosphate is a crucial intermediate in various metabolic pathways, such as the synthesis of glycerolipids and triglycerides, glycogenesis, glycolysis and gluconeogenesis. |
Cellular Location | Mitochondrion outer membrane; Single-pass membrane protein. Nucleus. Cytoplasm, cytosol. Note=Glycerol kinase activity is more cytosolic in some tissues. It probably represents the expression of isoforms lacking a transmembrane domain [Isoform 4]: Cytoplasm, cytosol. Note=In adult tissues, such as liver the glycerol kinase activity is more cytosolic. It probably represents the expression of this isoform which lacks a transmembrane domain |
Tissue Location | [Isoform 2]: Widely expressed in fetal and adult tissues. [Isoform 4]: The sole isoform expressed in adult liver and kidney. |
Research Areas
For Research Use Only. Not For Use In Diagnostic Procedures.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Key enzyme in the regulation of glycerol uptake and metabolism.

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