> 首页 > 产品 > 一抗 > 免疫学 > LAG3 Antibody (Center)

LAG3 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

标注"皇冠"的产品，是满足高质量标准的产品。
了解更多...

All lanes : Anti-LAG3 Antibody (Center) at 1:1000 dilution Lane 1: K562 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: M. liver whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size : 57kDa Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes : Anti-LAG3 Antibody (Center) at 1:1000 dilution Lane 1: K562 whole cell lysate Lane 2: RPMI 8226 whole cell lysate Lane 3: Mouse heart tissue lysate Lane 4: Jurkat whole cell lysate Lane 5: A2058 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size : 57kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Overlay histogram showing Jurkat cells stained with AP6987C (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP6987C, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
All lanes : Anti-LAG3 Antibody (Center) at 1:2000 dilution Lane 1: 293T/17 whole cell lysates Lane 2: human spleen lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
LAG3 Antibody (Center) (Cat. #AP6987c) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the LAG3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

产品详情
实验流程
背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, FC, E
Primary Accession	P18627
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Antigen Region	103-132 aa

Additional Information
Other Names	Lymphocyte activation gene 3 protein, LAG-3, Protein FDC, CD223, LAG3, FDC
Target/Specificity	This LAG3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-132 amino acids from the Central region of human LAG3.
Dilution	WB~~1:2000 IHC-P~~1:100~500 FC~~1:25 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	LAG3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.