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CAV3 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

Anti-CAV3 Antibody (N-term)at 1:500 dilution + human normal uterus lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 17 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of CAV3 antibody (N-term) (Cat.# AP6516a) in 293 cell line lysates (35ug/lane). CAV3 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human Skeletal muscle reacted with CAV3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow cytometric analysis of 293 cells using CAV3 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, FC, E
Primary Accession	P56539
Other Accession	P51638, Q3ZDQ5, P51637, Q2KI43
Reactivity	Human, Rat, Mouse
Predicted	Bovine, Mouse, Pig, Rat
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Antigen Region	5-31 aa

Additional Information
Other Names	Caveolin-3, M-caveolin, CAV3
Target/Specificity	This CAV3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 5-31 amino acids from the N-terminal region of human CAV3.
Dilution	WB~~1:1000 IHC-P~~1:100~500 FC~~1:10~50 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	CAV3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

CAV3 is a caveolin family member, which functions as a component of the caveolae plasma membranes found in most cell types. Caveolin proteins are proposed to be scaffolding proteins for organizing and concentrating certain caveolin-interacting molecules. Mutations identified in its gene lead to interference with protein oligomerization or intra-cellular routing, disrupting caveolae formation and resulting in Limb-Girdle muscular dystrophy type-1C (LGMD-1C), hyperCKemia or rippling muscle disease (RMD).

REFERENCES

Garg,V., Biochem. Biophys. Res. Commun. 385 (3), 472-477 (2009)
Cai,C., . Biol. Chem. 284 (23), 15894-15902 (2009)

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¥ 699.00

Cat# AP6516A

Size:

25 ul

50 µl

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¥ 699.00

¥ 1,250.00

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CAV3 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

BACKGROUND

REFERENCES

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