Anti-Tachykinin Receptor 1 Antibody

Rabbit polyclonal antibody to Tachykinin Receptor 1

Western blot analysis of Tachykinin Receptor 1 expression in HEK293T (A), A2780 (B), mouse spleen (C), mouse kidney (D), rat spleen (E), rat kidney (F) whole cell lysates.
Immunofluorescent analysis of Tachykinin Receptor 1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P25103
Other Accession	P30548
Reactivity	Human, Mouse, Rat, Chicken, Drosophila
Host	Rabbit
Clonality	Polyclonal
Calculated MW	46251 Da

Additional Information
Gene ID	6869
Other Names	NK1R; TAC1R; Substance-P receptor; SPR; NK-1 receptor; NK-1R; Tachykinin receptor 1
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Tachykinin Receptor 1. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000), IF/IC (1/100 - 1/500) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	TACR1
Synonyms	NK1R, TAC1R
Function	Receptor for the tachykinin substance P, also able to bind and respond to tachynins neurokinin A/substance K and neurokinin B/neuromedin-K (PubMed:1718267, PubMed:15452552). The rank order of affinity of this receptor to tachykinins is: substance P > neurokinin A/substance K > neurokinin B/neuromedin-K (PubMed:1718267). Substance P binding to its receptor triggers G protein-coupled receptor signaling via activation of phosphatidylinositol hydrolysis by phospholipase C. Substance P binding also triggers signaling via activation of adenylate cyclase activity which results in increased intracellular levels of cyclic AMP (cAMP) (By similarity).
Cellular Location	Cell membrane; Multi-pass membrane protein. Early endosome {ECO:0000250\|UniProtKB:P14600}. Note=After signaling cascade initiation, substance P binding results in receptor internalization and localization to early endosomes, followed by receptor recycling to the cell surface. {ECO:0000250\|UniProtKB:P14600}

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.