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Anti-14-3-3 theta/tau Antibody

Rabbit polyclonal antibody to 14-3-3 theta/tau

     
  • 1 - Anti-14-3-3 theta/tau Antibody AP60089
    Western blot analysis of 14-3-3 theta/tau expression in HEK293T (A), Hela (B), A2780 (C), mouse brain (D), mouse liver (E) whole cell lysates.
  • 2 - Anti-14-3-3 theta/tau Antibody AP60089
    Immunohistochemical analysis of 14-3-3 theta/tau staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • 3 - Anti-14-3-3 theta/tau Antibody AP60089
    Immunofluorescent analysis of 14-3-3 theta/tau staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF/IC, IHC
Primary Accession P27348
Other Accession P68254
Reactivity Human, Mouse, Rat, Rabbit, Pig, Chicken, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 27764 Da
Additional Information
Gene ID 10971
Other Names 14-3-3 protein theta; 14-3-3 protein T-cell; 14-3-3 protein tau; Protein HS1
Target/Specificity Recognizes endogenous levels of 14-3-3 theta/tau protein.
Dilution WB~~WB (1/500 - 1/1000), IHC (1/100 - 1/200), IF/IC (1/100 - 1/500)
IF/IC~~N/A
IHC~~WB (1/500 - 1/1000), IHC (1/100 - 1/200), IF/IC (1/100 - 1/500)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name YWHAQ
Function Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.
Cellular Location Cytoplasm. Note=In neurons, axonally transported to the nerve terminals
Tissue Location Abundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression in individuals with predominant lower motor neuron involvement
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human 14-3-3 theta/tau. The exact sequence is proprietary.

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