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Anti-Histamine H2 Receptor Antibody

Rabbit polyclonal antibody to Histamine H2 Receptor

     
  • 1 - Anti-Histamine H2 Receptor Antibody AP59581
    Western blot analysis of Histamine H2 Receptor expression in mouse kidney (A), rat liver (B) whole cell lysates.
  • 3 - Anti-Histamine H2 Receptor Antibody AP59581
    Immunofluorescent analysis of Histamine H2 Receptor staining in COLO205 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF/IC
Primary Accession P25021
Other Accession P97292
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 40098 Da
Additional Information
Gene ID 3274
Other Names Histamine H2 receptor; H2R; HH2R; Gastric receptor I
Target/Specificity Recognizes endogenous levels of Histamine H2 Receptor protein.
Dilution WB~~WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
IF/IC~~N/A
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name HRH2
Function The H2 subclass of histamine receptors mediates gastric acid secretion. Also appears to regulate gastrointestinal motility and intestinal secretion. Possible role in regulating cell growth and differentiation. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and, through a separate G protein-dependent mechanism, the phosphoinositide/protein kinase (PKC) signaling pathway (By similarity).
Cellular Location Cell membrane; Multi-pass membrane protein.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Histamine H2 Receptor. The exact sequence is proprietary.

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