BCAN Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

BCAN Antibody (C-term) (Cat. #AP5915b) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the BCAN antibody detected the BCAN protein (arrow).
BCAN antibody (C-term) (Cat. #AP5915b) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of the BCAN antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
BCAN Antibody (C-term) (Cat. #AP5915b) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, FC, E
Primary Accession	Q96GW7
Other Accession	Q28062, NP_940819.1, NP_068767.3
Reactivity	Human
Predicted	Bovine
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	99118 Da
Antigen Region	715-741 aa

Additional Information
Gene ID	63827
Other Names	Brevican core protein, Brain-enriched hyaluronan-binding protein, BEHAB, Chondroitin sulfate proteoglycan 7, BCAN, BEHAB, CSPG7
Target/Specificity	This BCAN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 715-741 amino acids from the C-terminal region of human BCAN.
Dilution	WB~~1:1000 IHC-P~~1:100~500 FC~~1:10~50 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	BCAN Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	BCAN
Synonyms	BEHAB, CSPG7
Function	May play a role in the terminally differentiating and the adult nervous system during postnatal development. Could stabilize interactions between hyaluronan (HA) and brain proteoglycans.
Cellular Location	Secreted [Isoform 2]: Membrane; Lipid-anchor, GPI-anchor.
Tissue Location	Expressed in the retina, specifically in the inner nuclear layer, inner plexiform layer and ganglion cell layer (at protein level) (PubMed:29777959). Detected in cerebrospinal fluid (at protein level) (PubMed:25326458). Detected in urine (at protein level) (PubMed:37453717).

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.