Phospholipase D1/PLD1 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, IHC-F, IF, ICC, E
Primary Accession	Q13393
Reactivity	Rat, Pig, Dog, Bovine
Host	Rabbit
Clonality	Polyclonal
Calculated MW	124184 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human Phospholipase D1
Epitope Specificity	601-700/1074
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4.
SUBCELLULAR LOCATION	Cytoplasm > perinuclear region. Endoplasmic reticulum membrane. Golgi apparatus membrane. Late endosome membrane.
SIMILARITY	Belongs to the phospholipase D family. Contains 1 PH domain. Contains 2 PLD phosphodiesterase domains. Contains 1 PX (phox homology) domain.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Additional Information
Gene ID	5337
Other Names	Phospholipase D1, PLD 1, hPLD1, 3.1.4.4, Choline phosphatase 1, Phosphatidylcholine-hydrolyzing phospholipase D1, PLD1 (HGNC:9067)
Target/Specificity	Expressed abundantly in the pancreas and heart and at high levels in brain, placenta, spleen, uterus and small intestine.
Dilution	IHC-P=1:100-500,IHC-F=1:100-500,ICC=1:100-500,IF=1:100-500,ELISA=1:5000-10000
Format	0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Protein Information
Name	PLD1 (HGNC:9067)
Function	Function as phospholipase selective for phosphatidylcholine (PubMed:25936805, PubMed:8530346, PubMed:9582313). Implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic (By similarity).
Cellular Location	Cytoplasm, perinuclear region {ECO:0000250\|UniProtKB:Q9Z280}. Endoplasmic reticulum membrane {ECO:0000250\|UniProtKB:Q9Z280}; Lipid-anchor {ECO:0000250\|UniProtKB:Q9Z280}; Cytoplasmic side {ECO:0000250\|UniProtKB:Q9Z280}. Golgi apparatus membrane {ECO:0000250\|UniProtKB:Q9Z280}; Lipid-anchor {ECO:0000250\|UniProtKB:Q9Z280}; Cytoplasmic side {ECO:0000250\|UniProtKB:Q9Z280}. Late endosome membrane {ECO:0000250\|UniProtKB:Q9Z280}; Lipid-anchor {ECO:0000250\|UniProtKB:Q9Z280}; Cytoplasmic side {ECO:0000250\|UniProtKB:Q9Z280}
Tissue Location	Expressed abundantly in the pancreas and heart and at high levels in brain, placenta, spleen, uterus and small intestine