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GPR65 Rabbit pAb
GPR65 Rabbit pAb
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- 实验流程
- 背景知识
Application 
| WB |
|---|---|
| Primary Accession | Q8IYL9 |
| Reactivity | Rat, Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 39333 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from human GPR65 |
| Epitope Specificity | 51-120/337 |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SUBCELLULAR LOCATION | Cell membrane. |
| SIMILARITY | Belongs to the G-protein coupled receptor 1 family. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | GPR65 is a member of the G protein coupled receptor family. It has been reported in human in peripheral blood leukocytes, spleen, lymph node, and thymus. The ligand for this protein is psychosine. GPR65 may have a role in activation-induced cell death or differentiation of T cells. |
| Gene ID | 8477 |
|---|---|
| Other Names | G-protein coupled receptor 65, Psychosine receptor, T-cell death-associated gene 8 protein, GPR65 {ECO:0000303|PubMed:27287411, ECO:0000312|HGNC:HGNC:4517} |
| Target/Specificity | Predominantly expressed in thymus, spleen, lymph nodes, small intestine, lung, placenta and peripheral blood leukocytes. |
| Dilution | WB=1:500-2000 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| Name | GPR65 {ECO:0000303|PubMed:27287411, ECO:0000312|HGNC:HGNC:4517} |
|---|---|
| Function | Proton-sensing G-protein coupled receptor activated by extracellular pH, which is required to monitor pH changes and generate adaptive reactions (PubMed:15326175, PubMed:15618224, PubMed:20855608, PubMed:33478938, PubMed:37722051, PubMed:39753132). Activated by an optimal pH of 7.4 (PubMed:39753132). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide- binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase (PubMed:15326175, PubMed:15618224, PubMed:37722051, PubMed:39753132). GPR65 is mainly coupled to G(s) G proteins and mediates activation of adenylate cyclase activity (PubMed:15618224, PubMed:37722051, PubMed:39753132). May also act as a receptor for the glycosphingolipid psychosine (PSY) and several related glycosphingolipids (PubMed:11309421, PubMed:15326175). Plays a role in immune response by maintaining lysosome function and regulating T-cell metabolism (PubMed:27287411). Acts as a regulator of inflammation by mediating pH-sensing of extracellular acidification which takes place in inflamed tissues: activation regulates endo-lysosomal function of immune cells and T-cell metabolism (By similarity). Constitutively active in endosomes and stimulates adenylate cyclase production from endosomes independently from extracellular pH changes (PubMed:39753132). |
| Cellular Location | Cell membrane; Multi-pass membrane protein. Early endosome membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Note=Internalizes and localizes to early and late endosomes, from where GPR65 signals at steady state, irrespective of extracellular pH (PubMed:39753132). Changes in extracellular pH may relocalize receptor signaling to the cell membrane (PubMed:39753132). |
| Tissue Location | Predominantly expressed in thymus, spleen, lymph nodes, small intestine, lung, placenta and peripheral blood leukocytes |
Research Areas
For Research Use Only. Not For Use In Diagnostic Procedures.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
GPR65 is a member of the G protein coupled receptor family. It has been reported in human in peripheral blood leukocytes, spleen, lymph node, and thymus. The ligand for this protein is psychosine. GPR65 may have a role in activation-induced cell death or differentiation of T cells.
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