Contactin 3 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Contactin 3 Polyclonal Antibody, Unconjugated(AP58365) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, IHC-F, IF, E
Primary Accession	Q9P232
Reactivity	Rat, Dog, Bovine
Host	Rabbit
Clonality	Polyclonal
Calculated MW	112883 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human Contactin 3
Epitope Specificity	301-400/1028
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Cell membrane; Lipid-anchor, GPI-anchor.
SIMILARITY	Belongs to the immunoglobulin superfamily. Contactin family.Contains 4 fibronectin type-III domains.Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	Contactins mediate cell surface interactions during nervous system development. Contactin 3 has some neurite outgrowth-promoting activity.

Additional Information
Gene ID	5067
Other Names	Contactin-3, Brain-derived immunoglobulin superfamily protein 1, BIG-1, Plasmacytoma-associated neuronal glycoprotein, CNTN3, KIAA1496, PANG
Target/Specificity	In brain, it is expressed in frontal lobe, occipital lobe, cerebellum and amygdala.
Dilution	IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
Format	0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Protein Information
Name	CNTN3
Synonyms	KIAA1496, PANG
Function	Contactins mediate cell surface interactions during nervous system development. Has some neurite outgrowth-promoting activity (By similarity).
Cellular Location	Cell membrane; Lipid-anchor, GPI- anchor
Tissue Location	In brain, it is expressed in frontal lobe, occipital lobe, cerebellum and amygdala.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.