DEGS1 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF, E
Primary Accession	O15121
Reactivity	Rat, Pig, Bovine
Host	Rabbit
Clonality	Polyclonal
Calculated MW	37866 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human DEGS1
Epitope Specificity	21-120/323
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Mitochondrion. Endoplasmic reticulum membrane; Multi-pass membrane protein.
SIMILARITY	Belongs to the fatty acid desaturase family. DEGS subfamily.
Post-translational modifications	Myristoylation can target the enzyme to the mitochondria leading to an increase in ceramide levels.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	DEGS1 is a member of the membrane fatty acid desaturase family which is responsible for inserting double bonds into specific positions in fatty acids. It contains three His containing consensus motifs that are characteristic of a group of membrane fatty acid desaturases. It has sphingolipid-delta-4-desaturase activity and converts D-erythro-sphinganine to D-erythro-sphingosine (E-sphing-4-enine).

Additional Information
Gene ID	8560
Other Names	Sphingolipid delta(4)-desaturase DES1, 1.14.19.17, Cell migration-inducing gene 15 protein, Degenerative spermatocyte homolog 1, Dihydroceramide desaturase-1, Membrane lipid desaturase, DEGS1 (HGNC:13709), DES1, MLD
Target/Specificity	Ubiquitous.
Dilution	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
Format	0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Protein Information
Name	DEGS1 (HGNC:13709)
Synonyms	DES1, MLD
Function	Has sphingolipid-delta-4-desaturase activity. Converts D- erythro-sphinganine to D-erythro-sphingosine (E-sphing-4-enine) (PubMed:11937514, PubMed:30620337, PubMed:30620338). Catalyzes the equilibrium isomerization of retinols (By similarity).
Cellular Location	Mitochondrion membrane. Endoplasmic reticulum membrane; Multi-pass membrane protein
Tissue Location	Ubiquitous..