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KAAG1 Rabbit pAb

KAAG1 Rabbit pAb

     
  • 0 - KAAG1 Rabbit pAb AP56611
    Blank control: A549.
    Primary Antibody (green line): Rabbit Anti-KAAG1 antibody (AP56611)
    Dilution: 1 µg /10^6 cells;
    Isotype Control Antibody (orange line): Rabbit IgG .
    Secondary Antibody : Goat anti-rabbit IgG-PE
    Dilution: 1 µg /test.
    Protocol
    The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
  • 4 - KAAG1 Rabbit pAb AP56611
    Blank control: A549.
    Primary Antibody (green line): Rabbit Anti-KAAG1 antibody (AP56611)
    Dilution: 1 µg /10^6 cells;
    Isotype Control Antibody (orange line): Rabbit IgG .
    Secondary Antibody : Goat anti-rabbit IgG-PE
    Dilution: 1 µg /test.
    Protocol
    The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Product Information
Primary Accession Q9UBP8
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 8969 Da
Physical State Liquid
Immunogen KLH conjugated synthetic peptide derived from human KAAG1
Epitope Specificity 31-84/84
Isotype IgG
Purity affinity purified by Protein A
Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Additional Information
Other Names Kidney-associated antigen 1, RU2 antisense gene protein, KAAG1, RU2AS
Target/Specificity Expressed in testis and kidney, and, at lower levels, in urinary bladder and liver. Expressed by a high proportion of tumors of various histologic origin, including melanomas, sarcomas and colorectal carcinomas.
Dilution Flow-Cyt=1ug/Test
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Protein Information
Name KAAG1
Synonyms RU2AS
Tissue Location Expressed in testis and kidney, and, at lower levels, in urinary bladder and liver. Expressed by a high proportion of tumors of various histologic origin, including melanomas, sarcomas and colorectal carcinomas.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

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